Ecarin is a snake venom (Echis carinatus) that directly activates prothrombin to meizothrombin This action is not dependent on phospholipid membranes and . Objective(s): Echis carinatus is one of the venomous snakes in Iran. The venom of Iranian Echis carinatus is a rich source of protein with various factors affecting . In this research, the effects of Echis carinatus crude venom and its fractions on mice were analyzed. Moreover, the results of coagulation tests.

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Clinical concerns in snakebite include types of coagulopathy procoagulant, anticoagulant, fibrinogen clotting, fibrinolytic, platelet-active, thrombotic, hemorrhagicdiagnosis and treatment vrnom 1 – 3 ].

A Dissected skins of guinea pigs showing oral administrations of the H. Mouse blood samples were centrifuged for ten minutes at 3, rpm.

Approaching the golden age of natural product pharmaceuticals from venom libraries: Echis carinatus is one of the venomous snakes in Echiw. Purification and carinahus of prothrombin activator from the venom of Echis carinatus. The bitten limb must not be exercised as muscular contraction will promote systemic absorption of venom.

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Isolation and purification of coagulation factors Isolation and purification of coagulation factors were performed using 50 mg of Ec crude venom using a combination of gel chromatography and ion exchange chromatography. Viperidae venoms, including that of Ec, are rich in compounds that may be useful for medicine and pharmaceutics [ 20 ]. Prothrombin time test on mouse plasma by using fractions obtained from gel chromatography. Ion exchange chromatography of F 1 fraction.

Snake venom of Echis carinatus sochureki – Latoxan

InHowes et al. For more investigation into the coagulation activity, these subfractions were selected for injection into mice.


Author information Article notes Copyright and License information Disclaimer. Integrated Taxonomic Information System. Prothrombin time PT test The thromboplastin-D vial was brought to the cafinatus and equilibrated to room temperature. Pseudoboa carinatanew species, pp.

WCH Clinical Toxinology Resources

The total protein of crude venom of E. These include local cauterization, incision, excision, amputation, suction by mouth, vacuum pump or syringe, combined incision and suction “venom-ex” apparatusinjection or instillation of compounds such as potassium permanganate, phenol carbolic soap and trypsin, application of electric shocks or ice cryotherapyuse of traditional herbal, folk and other remedies including the ingestion of emetic plant products and parts of the snake, multiple incisions, tattooing and so on.

The process of plasma clotting was observed and the time recorded [ 11 ]. The present study reports an efficient and simple procedure for purification and isolation of procoagulant factor from I Ec venom. Statistical results suggest that H 0 is rejected by both subfractions F 1 A and F 1 B and, hypothetically, H 1 is accepted by both.

The present study showed that the crude venom of E.

Snake venom of Echis carinatus sochureki

Our observation showed that the molecular weight of F 1 B 4 is similar to prothrombin activator enzymes which have been already reported Carinatus 24aharin from Agkistrodon halys pallas 25 and prothrombin activator from Bothrops atrox Ec, a native species in Iran, has been killed by people for a long time because of its bites. Over the past 20 years, many metalloproteinase have been isolated from snake venom with a wide variety of biological activities, including hemorrhagic 27fibrinogenolytic and antiplatelet effects 28as well as activation of prothrombin and factor X Accessed 15 August Although an intravenous administration of antivenom, prepared from IgG of venomimmunised horses or sheep, is an effective treatment for systemic envenoming, the clinical consensus is that antivenom is of limited effectiveness against the effects of local envenoming that develop rapidly after a bite.


Group 2 for inhibition examination was divided into 8 equal subgroups 3 guinea pig in each were injected subcutaneously with the venom sample that was preincubated with the extract at different concentrations Echis carinatuspp. However, it showed coagulation effects on rabbit fibrinogen [ 2829 ].

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Prothrombin-like enzymes used in defibrillation are part of the thrombolytic treatment clotting decomposition. Neurotoxic Paralysis Does not occur, based on current clinical evidence.

Compared with the normal time, this interval is lower, showing the intense coagulation properties of these subfractions. Venom constitution within the same snake species can present considerable geographical variations [ 10 — 12 ], including a diverse in conformity with interpopulational, seasonal, ontogenetic, and individual factors [ 13 ]. Accessed 6 January Echis carinatus Saw scaled viper is a venomous snake found in the desert regions of Iran.

This coagulation difference may be indirectly attributed to failure and dysfunction in blood coagulation factors by the presence of procoagulation compounds, such as prothrombin activators. From Uzbekistan to Iran in the south and east to western Pakistan.

They also led to disturbances in fibrin formation and caused systemic bleeding. Samples of the crude venom and its fractions were lauded and the molecular weights of protein were determined under reduced conditions. Hematemesismelenahemoptysishematuria and epistaxis also occur and may lead to hypovolemic shock.

In vitro effects of Echis carinatus venom on the human plasma proteome. No abnormal behaviour was observed. Blood coagulation induced by the venom of Bothrops atrox.